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Research Associate II - Cell Biologist

Company: Takeda Pharmaceutical
Location: Chula Vista
Posted on: August 4, 2022

Job Description:

By clicking the "Apply" button, I understand that my employment application process with Takeda will commence and that the information I provide in my application will be processed in line with Takeda s Privacy Notice and Terms of Use . I further attest that all information I submit in my employment application is true to the best of my knowledge. Job Description About the role: At Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.Join Takeda as a Research Associate II - Cell Biologist where you will perform routine lab assignments and protocols; contribute to design of and implementation of improvements for routine procedures. How you will contribute:

  • You will culture and differentiate iPSCs to terminal cell types in 2-D or 3-D culture formats
  • You will evaluate and troubleshoot differentiation protocols for cell manufacturing: cell culture and cell engineering process development and optimization, scaling, QC, SOP writing and tech transfer
  • Perform phenotypic characterization analysis: flow cytometry, immunofluorescence, ELISA, cell-based assays, and gene expression
  • Produce cells required for pre-clinical studies.
  • Under minimal supervision, you will also perform multiple assigned research experiments with efficiency, accuracy and attention to detail (e.g. selects methods, troubleshoots routine and some non-routine procedures)
  • Exercise technical judgement in execution of specialized procedures and interpretation of complex results
  • Initiate routine and non-routine problem investigation and recommend solutions
  • Culture and differentiation of human iPSCs and iPSC-derived cells (2D/3D)
  • Experimental design for flow cytometry, qPCR, immunofluorescence, or functional assays
  • Develop robust methods to support GMP-compatible cell engineering workstreams
  • Follow, troubleshoot, and produce SOPs
  • Production of cells for preclinical studies
  • Analysis of experimental data using standard software packages
  • Detailed record keeping and ability to effectively communicate experimental results to team members
  • Develop knowledge of cell engineering methods
  • Record keeping of data and inventories of samples and cell banks
  • Collaborate with other members of the DDU for assay development and validation
  • Engage with vendors and external service providers
  • Attendance at scientific conferences Minimum Requirements/Qualifications:
    • BS in a scientific discipline (or equivalent) with 2+ years experience, or MS in a scientific discipline
    • Demonstrate technical knowledge of a scientific discipline
    • Understand specific scientific mechanisms of why a procedure works
    • Experience culturing and differentiating human cell types in 2-D or 3-D formats, including adherent and bioreactor culture processes
    • Hands-on experience with FACS/flow cytometry methods is desirable
    • Experience with RNA isolation, qRT-PCR, immunofluorescence, ELISA, and common in vitro cellular analysis methods
    • Experience with methods for gene editing, plasmid design, and molecular cloning is desirable
    • Experience in immunology and immune cell culture is desirable
    • Use common software packages to analyze data sets (GraphPad Prism, Excel, FlowJo)
    • Experience in GMP process is a plus
    • Experience drafting SOPs and batch records is a plus What Takeda can offer you:
      • Comprehensive Healthcare: Medical, Dental, and Vision
      • Financial Planning & Stability: 401(k) with company match and Annual Retirement Contribution Plan
      • Health & Wellness programs including onsite flu shots and health screenings
      • Generous time off for vacation and the option to purchase additional vacation days
      • Community Outreach Programs and company match of charitable contributions
      • Family Planning Support
      • Flexible Work Paths
      • Tuition reimbursement More about us: At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs. Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work.Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.In accordance with the CO Equal Pay Act, Colorado Applicants Are Not Permitted to Apply.EEO Statement Takeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law. Locations San Diego, CA Worker Type Employee Worker Sub-Type Regular Time Type Full time

Keywords: Takeda Pharmaceutical, Chula Vista , Research Associate II - Cell Biologist, Other , Chula Vista, California

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